Tg(Igh-6-cre/ERT)30Afst
Transgene Detail

Summary

• Symbol: Tg(Igh-6-cre/ERT)30Afst
• Name: transgene insertion 30, A Francis Stewart
• MGI ID: MGI:3702034
• Synonyms: Cre-EBD(G521R), CreED-30, Tg(Igh-6-cre/ESR1)30Afst
• Transgene: Tg(Igh-6-cre/ERT)30Afst Location: unknown
• Alliance: Tg(Igh-6-cre/ERT)30Afst page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Igh-6-cre/ERT)30Afst expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Igh

• Mutation details: The transgene was created by combining the Igh-6 enhancer with a minimal SV40 promoter to drive the expression of a cre-ERT mutant fusion protein. The fusion protein consisted of cre recombinase fused to the estrogen binding domain of the human estrogen receptor mutated at amino acid 521 (G521R). Expression in ~ 75% of peripheral blood B lymphocytes was confirmed with an anti-cre antibody. (J:69333)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Igh (species not specified) Summary of all recombinase alleles driven by Igh.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Line 30 is a representative expressing line; line 24 was also created with a similar expression pattern.

References

• Original: J:69333 Schwenk F, et al., Temporally and spatially regulated somatic mutagenesis in mice. Nucleic Acids Res. 1998 Mar 15;26(6):1427-32
• All: 6 reference(s)