Col1a1^{tm2(tetO-Pou5f1)Jae}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(tetO-Pou5f1)Jae}
• Name: collagen, type I, alpha 1; targeted mutation 2, Rudolf Jaenisch
• MGI ID: MGI:3702325
• Synonyms: Col1a1::TetOP-Oct4, TetON-Oct4
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(tetO-Pou5f1)Jae} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98920
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm2(tetO-Pou5f1)Jae} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pou5f1 (MGI:101893)

• Mutation details: ES cells containing the Gt(ROSA)26Sor^{tm1(rtTA2S-M2)Jae} mutation were retargeted. Cells were injected with a "flip-in plasmid" containing a tetracycline responsive element (TRE or tetO) and mouse Oct4 (Pou5f1) cDNA sequence (and a Flpe-recombinase plasmid to facilitate tetO-Oct4 integration into the 3' UTR region of Col1a1). With doxycycline treatment, Pou5f1 expression from the Col1a1 locus is widespread with high levels in skin, intestine and stomach, while lower levels are seen in the heart, spleen, thymus and several other organs. No expression is seen in the brain or testes. (J:98920)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:98920 Hochedlinger K, et al., Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell. 2005 May 6;121(3):465-77
• All: 10 reference(s)