Tcrb^tm2Slek
Targeted Allele Detail

Summary

• Symbol: Tcrb^tm2Slek
• Name: T cell receptor beta chain; targeted mutation 2, Barry P Sleckman
• MGI ID: MGI:3702574
• Synonyms: Jbeta1^M4
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119552
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: ES cells containing Tcrb^tm1Slek, which contain a loxP site in place of the Dbeta2-Jbeta2 gene cluster, were additionally targeted to replace the 5' Dbeta1 12-RSS with the Jbeta1.2 12-RSS. The allele also contains loxP and HindIII sites between Dbeta1 and Jbeta1.1, as well as the conversion of a bamHI site 3' of Jbeta1.2 to a PstI site. A floxed neo cassette was removed from the locus. (J:119552)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 97 strains or lines available

References

• Original: J:119552 Bassing CH, et al., Recombination signal sequences restrict chromosomal V(D)J recombination beyond the 12/23 rule. Nature. 2000 Jun 1;405(6786):583-6
• All: 4 reference(s)