Cybrd1^tm1Nca
Targeted Allele Detail

Summary

• Symbol: Cybrd1^tm1Nca
• Name: cytochrome b reductase 1; targeted mutation 1, Nancy C Andrews
• MGI ID: MGI:3702743
• Synonyms: Cybrd1^-
• Gene: Cybrd1 Location: Chr2:70948398-70973270 bp, + strand Genetic Position: Chr2, 42.38 cM, cytoband C3
• Alliance: Cybrd1^tm1Nca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119528
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed to insert a loxP site into intron 1 and a loxP-flanked neo/cytosine deaminase cassette into intron 2. Transient expression of cre recombinase resulted in the removal of exon 2. Immunoblot confirmed absence of protein in mutant duodenal lysates. (J:119528)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cybrd1 Mutation: 18 strains or lines available

References

• Original: J:119528 Gunshin H, et al., Cybrd1 (duodenal cytochrome b) is not necessary for dietary iron absorption in mice. Blood. 2005 Oct 15;106(8):2879-83
• All: 1 reference(s)