Helt^tm1Wrst
Targeted Allele Detail

Summary

• Symbol: Helt^tm1Wrst
• Name: helt bHLH transcription factor; targeted mutation 1, Wolfgang Wurst
• MGI ID: MGI:3703237
• Synonyms: Mgn^tZ
• Gene: Helt Location: Chr8:46745076-46747708 bp, - strand Genetic Position: Chr8, 26.32 cM
• Alliance: Helt^tm1Wrst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119562
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to delete the bHLHO domain, which is encoded by exons 2 and 3. A tau-lacZ fusion cassette was inserted in frame with the 12th codon in exon 2, followed by a floxed neomycin resistance cassette. The neo was subsequently removed by cre-mediated recombination. Absence of transcript was confirmed by RT-PCR. Expression of the mutant protein was confirmed by in situ hybridization. (J:119562)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Helt Mutation: 9 strains or lines available

References

• Original: J:119562 Guimera J, et al., Megane/Heslike is required for normal GABAergic differentiation in the mouse superior colliculus. Development. 2006 Oct;133(19):3847-57
• All: 5 reference(s)