Neb^tm1Slbt
Targeted Allele Detail

Summary

• Symbol: Neb^tm1Slbt
• Name: nebulin; targeted mutation 1, Siegfried Labeit
• MGI ID: MGI:3703320
• Synonyms: NEB KO
• Gene: Neb Location: Chr2:52026652-52228810 bp, - strand Genetic Position: Chr2, 29.98 cM
• Alliance: Neb^tm1Slbt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119578
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The cap site, TATA-box and its putative Tmod-binding aminoterminal domain in the 5' UTR were replaced with a neomycin resistance cassette (also known as exon 2 and 3 in later reports). Western blot revealed absence of transcript in mutant mice. (J:119578)

Disease models

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Neb Mutation: 401 strains or lines available

References

• Original: J:119578 Witt CC, et al., Nebulin regulates thin filament length, contractility, and Z-disk structure in vivo. EMBO J. 2006 Aug 23;25(16):3843-55
• All: 10 reference(s)