Lemd3^{Gt(XST167)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Lemd3^{Gt(XST167)Byg}
• Name: LEM domain containing 3; gene trap XST167, BayGenomics
• MGI ID: MGI:3703328
• Synonyms: Man1^delta
• Gene: Lemd3 Location: Chr10:120759318-120815237 bp, - strand Genetic Position: Chr10, 68.88 cM
• Alliance: Lemd3^{Gt(XST167)Byg} page

Mutation origin

• Mutant Cell Line: XST167
• Germline Transmission: Earliest citation of germline transmission: J:119566
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: The pGT1Mpfs gene trap vector inserted into intron 4 of the locus, deleting the C-terminal region containing the Smad-interaction domain. Western blot failed to detect wild-type protein from mutants. (J:119566)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XST167	CC178867 ; (MGI Seq Detail)	5' RACE	Chr10:120783108-120813959 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	44 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lemd3 Mutation: 36 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:119566 Ishimura A, et al., Man1, an inner nuclear membrane protein, regulates vascular remodeling by modulating transforming growth factor beta signaling. Development. 2006 Oct;133(19):3919-28
• All: 4 reference(s)