Tg(APOC3)2674Bres
Transgene Detail

Summary

• Symbol: Tg(APOC3)2674Bres
• Name: transgene insertion 2674, Jan L Breslow
• MGI ID: MGI:3703623
• Synonyms: Low exp (2674), low expressor, Tg line 2674
• Transgene: Tg(APOC3)2674Bres Location: unknown
• Alliance: Tg(APOC3)2674Bres page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(APOC3)2674Bres expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOC3 (345)

  Apoc3

  (MGI:88055)

• Mutation details: Male pronuclei of fertilized oocytes were microinjected with a transgenic construct comprising the human apolipoprotein C-III gene, including 2.5 kb of 5' and 1.1 kb of 3' flanking sequence. 1-2 copies of the transgene were estimated present per haploid genome by quantitative Southern blot analysis. Solution hybridization RNase protection analysis with a riboprobe representing the 3' untranslated region of human APOC3 demonstrated transgene-derived RNA in liver and intestine, but none in brain of transgenic mice. Electroimmunoanalysis with a human-specific anti-APOC3 antibody detected human APOC3 in plasma of mice hemizygous for the transgene at 3.15 +/- 0.3 mg/dl mice and in plasma of homozygous transgenic mice at 8.2 +/- 1.0 mg/dl. (J:81184)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:81184 Ito Y, et al., Hypertriglyceridemia as a result of human apo CIII gene expression in transgenic mice. Science. 1990 Aug 17;249(4970):790-3
• All: 3 reference(s)