Man1a2^tm1.1Ahe
Targeted Allele Detail

Summary

• Symbol: Man1a2^tm1.1Ahe
• Name: mannosidase, alpha, class 1A, member 2; targeted mutation 1.1, Annette Herscovics
• MGI ID: MGI:3703789
• Synonyms: Man1B^-
• Gene: Man1a2 Location: Chr3:100469519-100592789 bp, - strand Genetic Position: Chr3, 43.71 cM
• Alliance: Man1a2^tm1.1Ahe page

Thick interalveolar septae and alveolar hemorrhaging in E18.5 Man1a2^tm1.1Ahe/Man1a2^tm1.1Ahe lungs

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119977
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The floxed exon 2 in Man1a2^tm1Ahe was removed via transient transfection with Cre recombinase. Excision of the floxed exon causes a frameshift and introduces a stop codon immediately following exon 1. RT-PCR confirmed the loss of gene expression in various tissues. (J:119977)

Expression

In Mice Carrying this Mutation:	17 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Man1a2 Mutation: 32 strains or lines available

References

• Original: J:119977 Tremblay LO, et al., Respiratory distress and neonatal lethality in mice lacking Golgi alpha1,2-mannosidase IB involved in N-glycan maturation. J Biol Chem. 2007 Jan 26;282(4):2558-66
• All: 3 reference(s)