Tg(Ins2-Nos2)40Okam
Transgene Detail

Summary

• Symbol: Tg(Ins2-Nos2)40Okam
• Name: transgene insertion 40, Hiroshi Okamoto
• MGI ID: MGI:3706439
• Synonyms: line 40
• Transgene: Tg(Ins2-Nos2)40Okam Location: unknown
• Alliance: Tg(Ins2-Nos2)40Okam page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-Nos2)40Okam expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nos2 (MGI:97361)

• Tg(Ins2-Nos2)40Okam expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgenic construct was composed of the rat insulin II promoter and the 3.5 kb cDNA fragment containing the entire mouse Nos2 gene coding region with the SV40 intron/polyadenylation signal ligated to it. Line 31 was subsequently established. Western blot analysis shows expression of Nos2 in pancreatic islets, but nowhere else. (J:45520)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

J:116040 uses transgenic Nos2 mice with no identification of the line. The two lines created in J:45520 are similar, with line 40 having a more severe phenotype. Assumption was made that line 40 was used subsequently for this reason. rpb 05/01/2007

References

• Original: J:45520 Takamura T, et al., Transgenic mice overexpressing type 2 nitric-oxide synthase in pancreatic beta cells develop insulin-dependent diabetes without insulitis. J Biol Chem. 1998 Jan 30;273(5):2493-6
• All: 5 reference(s)