Hectd1^Gt(XC266)Byg
Gene trapped Allele Detail

Summary

• Symbol: Hectd1^Gt(XC266)Byg
• Name: HECT domain E3 ubiquitin protein ligase 1; gene trap XC266, BayGenomics
• MGI ID: MGI:3706565
• Synonyms: Hectd1^X, Hectd1^XC
• Gene: Hectd1 Location: Chr12:51790505-51876319 bp, - strand Genetic Position: Chr12, 22.11 cM
• Alliance: Hectd1^Gt(XC266)Byg page

Mutation origin

• Mutant Cell Line: XC266
• Germline Transmission: Earliest citation of germline transmission: J:122594
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap containing a lacZ-stop cassette was inserted into the HECT domain resulting in a protein truncation. Insertion was confirmed by genotyping using lacZ-specific primers and lacZ expression in a beta-galactosidase assay. (J:122594)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XC266	CC178227 ; (MGI Seq Detail)	5' RACE	Chr12:51806135-51810871 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  29 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hectd1 Mutation: 142 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:122594 Zohn IE, et al., The Hectd1 ubiquitin ligase is required for development of the head mesenchyme and neural tube closure. Dev Biol. 2007 Jun 1;306(1):208-21
• All: 6 reference(s)