Tg(CD2-TcraF5,CD2-TcrbF5)1Kio
Transgene Detail

Summary

• Symbol: Tg(CD2-TcraF5,CD2-TcrbF5)1Kio
• Name: transgene insertion 1, Dimitris Kioussis
• MGI ID: MGI:3706698
• Synonyms: F5, F5 TCR, TCR F5
• Transgene: Tg(CD2-TcraF5,CD2-TcrbF5)1Kio Location: unknown
• Alliance: Tg(CD2-TcraF5,CD2-TcrbF5)1Kio page

Transgene origin

• Strain of Origin: C57BL/10

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD2-TcraF5,CD2-TcrbF5)1Kio expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Constructs were created, with the cDNAs for both the alpha chain (Valpha4) and the beta chain (Vbeta11) of the T cell receptor from the mouse CTL F5 clone, inserted in the 5' UTR of the human CD2 minigene carrying a frameshift mutation in its coding region. The CD2 minigene provides an intron, the 3' LCR and a polyadenylation signal which are sufficient to direct expression of the transgenes. The constructs were coinjected into C57BL/10 oocytes. Transmission of the transgenes produced several lines, and Southern blot verified multiple copies of the alpha and beta chain genes in one line. The transgene is specific for specific for influenza virus NP peptide presented by H-2D^b. (J:113713)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CD2-TcraF5,CD2-TcrbF5)1Kio

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:113713 Mamalaki C, et al., Positive and negative selection in transgenic mice expressing a T-cell receptor specific for influenza nucleoprotein and endogenous superantigen. Dev Immunol. 1993;3(3):159-74
• All: 70 reference(s)