Cebpa^tm5Nerl
Targeted Allele Detail

Summary

• Symbol: Cebpa^tm5Nerl
• Name: CCAAT/enhancer binding protein alpha; targeted mutation 5, Claus Nerlov
• MGI ID: MGI:3707189
• Synonyms: Cebpa^deltaPHR, DeltaPHR
• Gene: Cebpa Location: Chr7:34818718-34821353 bp, + strand Genetic Position: Chr7, 21.02 cM
• Alliance: Cebpa^tm5Nerl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:120098
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An in-frame deletion of amino acids 180-194 resulted in the loss of S193 and the associated PHR Cdk2/4 interaction motif. Western blot confirmed that mutants produced proteins slightly smaller than, but equal in abundance to, the wild-type allele. (J:120098)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cebpa Mutation: 19 strains or lines available

References

• Original: J:120098 Pedersen TA, et al., Distinct C/EBPalpha motifs regulate lipogenic and gluconeogenic gene expression in vivo. EMBO J. 2007 Feb 21;26(4):1081-93
• All: 1 reference(s)