Mesp2^tm8Ysa
Targeted Allele Detail

Summary

• Symbol: Mesp2^tm8Ysa
• Name: mesoderm posterior 2; targeted mutation 8, Yumiko Saga
• MGI ID: MGI:3707530
• Synonyms: 3xFLAG-Mesp2delta326-330, F-Mesp2deltaD
• Gene: Mesp2 Location: Chr7:79460475-79463179 bp, + strand Genetic Position: Chr7, 45.18 cM
• Alliance: Mesp2^tm8Ysa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119678
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: These mice express the FLAG-tagged locus lacking amino acids 326-330, which comprise the degredation domain. A floxed neo included in the vector was subsequently removed via cre mediated recombination. Expression of the mutant transcript was similar to wild type in localization, however, levels were lower. Likewise, protein expression was lower, demonstrating that the allele is a hypomorph. Western blot confirmed absence of wild-type protein in mutants. (J:119678)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mesp2 Mutation: 30 strains or lines available

References

• Original: J:119678 Morimoto M, et al., Cooperative Mesp activity is required for normal somitogenesis along the anterior-posterior axis. Dev Biol. 2006 Dec 15;300(2):687-98
• All: 1 reference(s)