Chd7^{Gt(S20-7E1)Sor}
Gene trapped Allele Detail

Summary

• Symbol: Chd7^{Gt(S20-7E1)Sor}
• Name: chromodomain helicase DNA binding protein 7; gene trap S20-7E1, Philippe Soriano
• MGI ID: MGI:3707678
• Synonyms: Chd7^Gt, Chd7^Gt1Dmm, Chd7^{Gt(FHCRC-GT-S20-7E1)Sor}, Chd7^{Gt(ROSAFARY)1Dmm}
• Gene: Chd7 Location: Chr4:8690406-8867659 bp, + strand Genetic Position: Chr4, 3.68 cM
• Alliance: Chd7^{Gt(S20-7E1)Sor} page

Abnormal development in Chd7^{Gt(S20-7E1)Sor}/Chd7^{Gt(S20-7E1)Sor} mice and delayed turning in Chd7^{Gt(S20-7E1)Sor}/Chd7⁺ embryos

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: S20-7E1
• Germline Transmission: Earliest citation of germline transmission: J:119812
• Parent Cell Line: AK7.1 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: ROSAFARY
• Mutation details: The gene trap vector ROSAFARY was inserted between exon 1 and 2 creating an exon 1/lacZ fusion transcript and an hyg fusion transcript with the remainder of the coding region. The absence of the normal splice product and the production of the two mutant variants was confirmed by RT-PCR. (J:119812)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  FHCRC-GT-S20-7E1	CL706141 ; (MGI Seq Detail)	3' RACE	Chr4:8751326-8785603 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  25 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	220 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	39 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chd7 Mutation: 138 strains or lines available

References

• Original: J:119812 Hurd EA, et al., Loss of Chd7 function in gene-trapped reporter mice is embryonic lethal and associated with severe defects in multiple developing tissues. Mamm Genome. 2007 Feb;18(2):94-104
• All: 19 reference(s)