Foxp3^tm3Tch
Targeted Allele Detail

Summary

• Symbol: Foxp3^tm3Tch
• Name: forkhead box P3; targeted mutation 3, Talal A Chatila
• MGI ID: MGI:3707723
• Synonyms: Foxp3^deltaEGFP, Foxp3-GFP
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^tm3Tch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:120636
• Parent Cell Line: SCC10 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: EGFP cDNA followed by a stop codon was inserted in-frame after codon 396 in exon 11 of Foxp3 and a loxP-flanked PGK-Neo was inserted in exon 9 in the same orientation of Foxp3. A diphtheria toxin cassette for negative selection was included in the targeting construct. Correctly targeted chimeric males were crossed with Cre-deleter mice on the BALB/c background to remove the PGK-cassette. Mice express a mutant Foxp3/EGFP protein with the 33 amino acid C-terminal peptide residues including DNA- and transcription factor-binding and a nuclear localization sequence replaced by EGFP under control of the endogenous promoter (J:120636)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 58 strains or lines available

References

• Original: J:120636 Lin W, et al., Regulatory T cell development in the absence of functional Foxp3. Nat Immunol. 2007 Apr;8(4):359-68
• All: 18 reference(s)