Sphk2^tm1.1Cgh
Targeted Allele Detail

Summary

• Symbol: Sphk2^tm1.1Cgh
• Name: sphingosine kinase 2; targeted mutation 1.1, Shaun R Coughlin
• MGI ID: MGI:3708000
• Gene: Sphk2 Location: Chr7:45358891-45367426 bp, - strand Genetic Position: Chr7, 29.45 cM
• Alliance: Sphk2^tm1.1Cgh page

Impaired lymphocyte egress in Lyve1^{tm1.1(EGFP/cre)Cys}/Lyve1⁺ Sphk1^tm2Cgh/Sphk1^tm2Cgh Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:120783
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An FRT-flanked neo cassette with a single 3' loxP site was inserted between exons 1 and 2 and an additional loxP site was inserted downstream of exon 5. The neo cassette was subsequently removed through flp mediated recombination. In vivo Cre-mediated recombination removed exons 2 through 5 creating a null allele. The loss of transcript was confirmed by RT-PCR. (J:120783)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sphk2 Mutation: 38 strains or lines available

References

• Original: J:120783 Pappu R, et al., Promotion of lymphocyte egress into blood and lymph by distinct sources of sphingosine-1-phosphate. Science. 2007 Apr 13;316(5822):295-8
• All: 13 reference(s)