Spint1^{Gt(OST380474)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Spint1^{Gt(OST380474)Lex}
• Name: serine protease inhibitor, Kunitz type 1; gene trap OST380474, Lexicon Genetics
• MGI ID: MGI:3709554
• Gene: Spint1 Location: Chr2:119067841-119079995 bp, + strand Genetic Position: Chr2, 59.97 cM, cytoband E5
• Alliance: Spint1^{Gt(OST380474)Lex} page

Mutation origin

• Mutant Cell Line: OST380474
• Germline Transmission: Earliest citation of germline transmission: J:118798
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A Lexicon gene trap vector was inserted between exon 2 and 3 to create a null allele. The absence of a transcript was confirmed by RT-PCR and the absence of protein product was confirmed by immunohistochemistry with an anti-Spint1 antibody. (J:118798)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST380474	CG643014 ; (MGI Seq Detail)	3' RACE	Chr2:119075633-119076899 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

25 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Spint1 Mutation: 26 strains or lines available

Notes

See also, data as provided by Lexicon Genetics, Inc.

References

• Original: J:118798 Fan B, et al., Hepatocyte growth factor activator inhibitor-1 (HAI-1) is essential for the integrity of basement membranes in the developing placental labyrinth. Dev Biol. 2007 Mar 1;303(1):222-30
• All: 2 reference(s)