Fkbp5^{Gt(RRC236)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Fkbp5^{Gt(RRC236)Byg}
• Name: FK506 binding protein 5; gene trap RRC236, BayGenomics
• MGI ID: MGI:3710108
• Synonyms: Fkbp51^-
• Gene: Fkbp5 Location: Chr17:28617727-28705123 bp, - strand Genetic Position: Chr17, 14.66 cM
• Alliance: Fkbp5^{Gt(RRC236)Byg} page

Mutation origin

• Mutant Cell Line: RRC236
• Germline Transmission: Earliest citation of germline transmission: J:120960
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The pGT1Lxf gene trap vector that contains a splice acceptor and beta-geo was inserted into intron 4. Northern blot, quantitative RT-PCR and Western blot confirmed the expression of a fusion transcript and protein that lacked the peptidyl-prolyl cis/trans-isomerase-like domain and all three TPR domains critical for the protein's function. (J:120960)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRC236	CC200245 ; (MGI Seq Detail)	5' RACE	Chr17:28647327-28659977 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  178 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fkbp5 Mutation: 170 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:120960 Yong W, et al., Essential role for Co-chaperone Fkbp52 but not Fkbp51 in androgen receptor-mediated signaling and physiology. J Biol Chem. 2007 Feb 16;282(7):5026-36
• All: 7 reference(s)