Braf^tm1Wds
Targeted Allele Detail

Summary

• Symbol: Braf^tm1Wds
• Name: Braf transforming gene; targeted mutation 1, William D Snider
• MGI ID: MGI:3711006
• Synonyms: Braf^exon3f, Braf^f
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1Wds page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121660
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were inserted to flank exon 3 according to nomenclature in J:29234. An FRT-flanked neomycin resistance cassette was removed prior to ES cell injection. Deletion of exon 3 would cause a reading frame shift and introduce a translational stop codon at position 132, truncating the RBD, CRD and kinase domains. (J:121660)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Braf^tm1Wds

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Neurocardiofaciocutaneous syndromes ( J:144862)

References

• Original: J:121660 Zhong J, et al., Raf kinase signaling functions in sensory neuron differentiation and axon growth in vivo. Nat Neurosci. 2007 May;10(5):598-607
• All: 4 reference(s)