Cdc20^Gt(XE368)Byg
Gene trapped Allele Detail

Summary

• Symbol: Cdc20^Gt(XE368)Byg
• Name: cell division cycle 20; gene trap XE368, BayGenomics
• MGI ID: MGI:3711105
• Synonyms: Cdc20^gt
• Gene: Cdc20 Location: Chr4:118290098-118294540 bp, - strand Genetic Position: Chr4, 54.61 cM
• Alliance: Cdc20^Gt(XE368)Byg page

Mutation origin

• Mutant Cell Line: XE368
• Germline Transmission: Earliest citation of germline transmission: J:121535
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector contained a splice acceptor, lacZ and neo cassette and inserted into intron 9. The vector fused in-frame and prevented expression of only the last 59 amino acids of the locus. Western blot confirmed expression of a much larger mutant protein than that in wild-type. (J:121535)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE368	CC183520 ; (MGI Seq Detail)	5' RACE	Chr4:118290679-118293061 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cdc20^Gt(XE368)Byg

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdc20 Mutation: 30 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:121535 Li M, et al., Loss of Cdc20 causes a securin-dependent metaphase arrest in two-cell mouse embryos. Mol Cell Biol. 2007 May;27(9):3481-8
• All: 4 reference(s)