Runx1^{tm1(cre/Esr1*)Ims}
Targeted Allele Detail

Summary

• Symbol: Runx1^{tm1(cre/Esr1*)Ims}
• Name: runt related transcription factor 1; targeted mutation 1, Igor M Samokhvalov
• MGI ID: MGI:3711451
• Synonyms: Runx1^Cre, Runx1^CreER, Runx1^MER-Cre-MER, Runx1^tm1(cre)Ims
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^{tm1(cre/Esr1*)Ims} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121435
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Hypomorph, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Runx1^{tm1(cre/Esr1*)Ims} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Runx1 (MGI:99852)

• Mutation details: A targeting vector was designed to insert a tamoxifen-inducible cre recombinase flanked by two mutated estrogen receptors and a floxed neo into the Runx1 gene downstream of the proximal (P2) promoter. The neo was subsequently removed via exogenous cre expression. A subsequent reference states that the allele was determined to be hypomorphic, with homozygotes developing to term but dying in the first postnatal day. (J:121435, J:182232)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Runx1 (mouse) Summary of all recombinase alleles driven by Runx1. Comparative matrix view of recombinase activities Alliance mouse Runx1 gene page

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:121435 Samokhvalov IM, et al., Cell tracing shows the contribution of the yolk sac to adult haematopoiesis. Nature. 2007 Apr 26;446(7139):1056-61
• All: 17 reference(s)