Braf^tm1Mmcm
Targeted Allele Detail

Summary

• Symbol: Braf^tm1Mmcm
• Name: Braf transforming gene; targeted mutation 1, Martin McMahon
• MGI ID: MGI:3711771
• Synonyms: Braf^CA, Braf^CA-V600E, BRAF^F-V600E, BRAFV600E^ca
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1Mmcm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121715
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a loxP site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a floxed neo cassette. A modified exon 15 was engineered into the vector to encode Braf^V600E and a silent XbaI restriction site. RT-PCR confirmed that mutant transcript was expressed at the same level as wild-type, and that the V600E mutation was not detected. (J:121715)

Disease models

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Braf^tm1Mmcm

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

References

• Original: J:121715 Dankort D, et al., A new mouse model to explore the initiation, progression, and therapy of BRAFV600E-induced lung tumors. Genes Dev. 2007 Feb 15;21(4):379-84
• All: 194 reference(s)