Piwil4^tm1Ghan
Targeted Allele Detail

Summary

• Symbol: Piwil4^tm1Ghan
• Name: piwi-like RNA-mediated gene silencing 4; targeted mutation 1, Gregory J Hannon
• MGI ID: MGI:3712153
• Synonyms: Miwi2^-
• Gene: Piwil4 Location: Chr9:14613072-14651968 bp, - strand Genetic Position: Chr9, 4.25 cM
• Alliance: Piwil4^tm1Ghan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:120824
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Duplication, Insertion
• Piwil4^tm1Ghan involves 1 genes/genome features (Piwil4) View all
• Mutation details: A 10 kb vector including a PGK-puro, and a duplication of the genomic region encompassing exons 9-12 were inserted downstream of exon 12. This insertion was predicted to insert multiple in-frame stop codons and to produce a nonfunctional allele. RT-PCR confirmed the absence of transcript in mutants and is concluded to be either severely hypomorphic or a null allele. (J:120824)

Expression

In Mice Carrying this Mutation:

9 assay results 7 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Piwil4 Mutation: 49 strains or lines available

References

• Original: J:120824 Carmell MA, et al., MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev Cell. 2007 Apr;12(4):503-14
• All: 17 reference(s)