Igh^tm1Mnz
Targeted Allele Detail

Summary

• Symbol: Igh^tm1Mnz
• Name: immunoglobulin heavy chain complex; targeted mutation 1, Michel C Nussenzweig
• MGI ID: MGI:3712224
• Synonyms: B1-8^hi
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75653
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: PCR was used to introduce a point mutation conferring higher affinity 4-hydroxy-3-nitrophenyl)acetyl (NP)-binding to Igh-V (VHB1-8): TGG->TTG (codon 33 Trp->Leu). A targeting vector incorporating a 5' floxed neomycin cassette in addition to the point mutation was transfected into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. (J:75653)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:75653 Shih TA, et al., Role of antigen receptor affinity in T cell-independent antibody responses in vivo. Nat Immunol. 2002 Apr;3(4):399-406
• All: 71 reference(s)