Ncf4^tm2Pth
Targeted Allele Detail

Summary

• Symbol: Ncf4^tm2Pth
• Name: neutrophil cytosolic factor 4; targeted mutation 2, Phillip T Hawkins
• MGI ID: MGI:3712357
• Synonyms: P40^phox-
• Gene: Ncf4 Location: Chr15:78129001-78146780 bp, + strand Genetic Position: Chr15, 37.1 cM
• Alliance: Ncf4^tm2Pth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121820
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A translational stop codon was introduced at codon 73 in exon 3 in the targeting vector. The vector also contains both a tACE-Cre and neoR module. Recombination in ES cells introduced the stop codon into the Ncf4 sequence. When chimeras were bred, tACE promoter activity in testes promoted Cre/loxP-mediated deletion of the cassette, leaving 59 bp of foreign DNA. No Ncf4 protein can be detected by antibodies in homozygous mice. (J:121820)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncf4 Mutation: 23 strains or lines available

References

• Original: J:121820 Ellson CD, et al., Neutrophils from p40phox-/- mice exhibit severe defects in NADPH oxidase regulation and oxidant-dependent bacterial killing. J Exp Med. 2006 Aug 7;203(8):1927-37
• All: 12 reference(s)