Akap10^{Gt(RRC139)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Akap10^{Gt(RRC139)Byg}
• Name: A kinase anchor protein 10; gene trap RRC139, BayGenomics
• MGI ID: MGI:3712925
• Gene: Akap10 Location: Chr11:61762133-61821078 bp, - strand Genetic Position: Chr11, 37.96 cM
• Alliance: Akap10^{Gt(RRC139)Byg} page

Mutation origin

• Mutant Cell Line: RRC139
• Germline Transmission: Earliest citation of germline transmission: J:121838
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap vector was inserted into the locus after exon 12. This insertion truncated the protein C terminus and eliminated the last 51 amino acids that include its PKA-binding domain. Immunoblot of cardiac tissue from mutants confirmed the absence of protein. (J:121838)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRC139	CC200288 ; (MGI Seq Detail)	5' RACE	Chr11:61777527-61781103 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  20 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Akap10 Mutation: 38 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:121838 Tingley WG, et al., Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation. Proc Natl Acad Sci U S A. 2007 May 15;104(20):8461-6
• All: 2 reference(s)