Tg(Fabp4-Plin*)1Asg
Transgene Detail

Summary

• Symbol: Tg(Fabp4-Plin*)1Asg
• Name: transgene insertion 1, Andrew S Greenberg
• MGI ID: MGI:3713708
• Synonyms: Peri Adelta1-6
• Transgene: Tg(Fabp4-Plin*)1Asg Location: unknown
• Alliance: Tg(Fabp4-Plin*)1Asg page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Fabp4-Plin*)1Asg expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Plin1 (MGI:1890505)

• Mutation details: A mouse Plin cDNA that had serine-to-alanine mutations made in all 6 protein kinase A phosphorylation sites using recombinant adenoviruses (Muta-Gene Phagemid in vitro mutagenesis kit (Bio-Rad)). The cDNA was FLAG-tagged at the carboxy terminus via PCR and was placed in a pBluescript vector containing the Fabp2 enhancer/promoter region, the SV40 small tumor antigen splice site, and polyadenylation signal sequence. This construct was injected in C57BL/6 fertilized oocytes. Mice express the transgene almost exclusively in brown adipose tissue. (J:113735)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:113735 Miyoshi H, et al., Perilipin promotes hormone-sensitive lipase-mediated adipocyte lipolysis via phosphorylation-dependent and -independent mechanisms. J Biol Chem. 2006 Jun 9;281(23):15837-44
• All: 2 reference(s)