Sirt1^tm1.1Ygu
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm1.1Ygu
• Name: sirtuin 1; targeted mutation 1.1, Yansong Gu
• MGI ID: MGI:3713747
• Synonyms: SIRT1-, SirT1^ko
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm1.1Ygu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121948
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin cassette was inserted just upstream of exon 4 and a third loxP site was inserted just downstream. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. Exon 4 (encoding a conserved Sir2 motif) was then removed by cre-mediated recombination to create a null allele. Absence of protein product was confirmed by western blot analysis. (J:121948)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 81 strains or lines available

References

• Original: J:121948 Li H, et al., SirT1 modulates the estrogen-insulin-like growth factor-1 signaling for postnatal development of mammary gland in mice. Breast Cancer Res. 2007;9(1):R1
• All: 8 reference(s)