Nfix^tm1Aes
Targeted Allele Detail

Summary

• Symbol: Nfix^tm1Aes
• Name: nuclear factor I/X; targeted mutation 1, Albrecht E Sippel
• MGI ID: MGI:3714028
• Synonyms: Nfix^-, Nfix^lacZ
• Gene: Nfix Location: Chr8:85431341-85527086 bp, - strand Genetic Position: Chr8, 41.02 cM, cytoband C1-C2
• Alliance: Nfix^tm1Aes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122250
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace all but the first 4 bp of exon 2 with a lacZ-loxP-neo-loxP cassette. Transient cre expression removed the neo in ES cells. RT-PCR confirmed absence of transcript in mutant brain, liver and lung samples. (J:122250)

Disease models

Expression

In Structures Affected by this Mutation:

16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfix Mutation: 61 strains or lines available

References

• Original: J:122250 Driller K, et al., Nuclear factor I X deficiency causes brain malformation and severe skeletal defects. Mol Cell Biol. 2007 May;27(10):3855-3867
• All: 2 reference(s)