Nkd1^tm1Kwha
Targeted Allele Detail

Summary

• Symbol: Nkd1^tm1Kwha
• Name: naked cuticle 1; targeted mutation 1, Keith Wharton
• MGI ID: MGI:3714460
• Synonyms: nkd1^lacZ
• Gene: Nkd1 Location: Chr8:89247982-89321512 bp, + strand Genetic Position: Chr8, 43.36 cM, cytoband C4
• Alliance: Nkd1^tm1Kwha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122353
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-b-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Northern blot analysis of lung tissue and Western blot analysis of embronic tissue in homozygotes confirm lack of normal mRNA expression, while X-gal staining closely mimics dynamic expression pattern of normal Nkd1 mRNA. (J:122353)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nkd1 Mutation: 17 strains or lines available

References

• Original: J:122353 Zhang S, et al., Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol. 2007 Jun;27(12):4454-64
• All: 1 reference(s)