Nkd2^tm1Kwha
Targeted Allele Detail

Summary

• Symbol: Nkd2^tm1Kwha
• Name: naked cuticle 2; targeted mutation 1, Keith Wharton
• MGI ID: MGI:3714464
• Synonyms: nkd2^lacZ
• Gene: Nkd2 Location: Chr13:73966653-73995750 bp, - strand Genetic Position: Chr13, 40.15 cM, cytoband C1
• Alliance: Nkd2^tm1Kwha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122353
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a stop codon into exon 6 at amino acid 121, as well as replace exons 6-8 of the targeted gene with an internal ribosome entry site-b-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. RT-PCR analysis of brain tissue in homozygotes confirms lack of normal mRNA expression, while X-gal staining closely mimics dynamic expression pattern of normal Nkd2 mRNA. (J:122353)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nkd2 Mutation: 21 strains or lines available

References

• Original: J:122353 Zhang S, et al., Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol. 2007 Jun;27(12):4454-64
• All: 1 reference(s)