Erap1^tm1Luc
Targeted Allele Detail

Summary

• Symbol: Erap1^tm1Luc
• Name: endoplasmic reticulum aminopeptidase 1; targeted mutation 1, Luc Van Kaer
• MGI ID: MGI:3714520
• Synonyms: ERAP1^-
• Gene: Erap1 Location: Chr13:74787692-74841321 bp, + strand Genetic Position: Chr13, 40.48 cM, cytoband C1
• Alliance: Erap1^tm1Luc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122479
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace a 600 kb fragment from intron 4 up to the 5' end of exon 6 (which contains the sequence encoding the Zinc-binding motif in the putative active site of the endogenous protein) with a neomycin resistance cassette. RT-PCR verified absence of mRNA in splenocytes and no protein is detected in hepatocytes by immunoblot. (J:122479)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Erap1 Mutation: 59 strains or lines available

References

• Original: J:122479 Yan J, et al., In vivo role of ER-associated peptidase activity in tailoring peptides for presentation by MHC class Ia and class Ib molecules. J Exp Med. 2006 Mar 20;203(3):647-59
• All: 6 reference(s)