Syt5^tm2Sud
Targeted Allele Detail

Summary

• Symbol: Syt5^tm2Sud
• Name: synaptotagmin V; targeted mutation 2, Thomas C Sudhof
• MGI ID: MGI:3715453
• Synonyms: S9F, synaptotagmin-9^fl, Syt9^tm1Sud
• Gene: Syt5 Location: Chr7:4542764-4550540 bp, - strand Genetic Position: Chr7, 2.62 cM, cytoband A1
• Alliance: Syt5^tm2Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122459
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to fuse a signal peptide from rat neurexin 1 alpha followed by a GFP "emerald" cassette to the amino(N) terminus of exon 1, flank exon 2 with loxP sites, and insert an Flp-flanked neomycin resistance cassette in intron 2. Resultant mice were crossed with an FRT recombinase strain to remove the FLP-flanked neomycin cassette. Presence of the GFP fusion protein was detected by western blot analysis. (J:122459)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syt5 Mutation: 15 strains or lines available

References

• Original: J:122459 Xu J, et al., Synaptotagmin-1, -2, and -9: Ca(2+) sensors for fast release that specify distinct presynaptic properties in subsets of neurons. Neuron. 2007 May 24;54(4):567-81
• All: 4 reference(s)