Ccn1^tm2Lfl
Targeted Allele Detail

Summary

• Symbol: Ccn1^tm2Lfl
• Name: cellular communication network factor 1; targeted mutation 2, Lester F Lau
• MGI ID: MGI:3715733
• Synonyms: CCN1^dm
• Gene: Ccn1 Location: Chr3:145352731-145355736 bp, - strand Genetic Position: Chr3, 70.18 cM
• Alliance: Ccn1^tm2Lfl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119829
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Exon 1 and half of exon 2 were replaced by a DNA cassette containing the Cyr61 promoter, the dm (apoptosis defective mutant allele) open reading frame (containing the signal sequence, IGFBP, VWC, variable, TSP and CT domains with H1 and H2 sites of Cyr61) and a neo cassette. The dm open reading frame is defective in apoptotic synergism with TNFalpha. The absence of protein product was confirmed by western blot analysis following serum-stimulation. (J:119829)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ccn1 Mutation: 35 strains or lines available

References

• Original: J:119829 Chen CC, et al., Cytotoxicity of TNFalpha is regulated by integrin-mediated matrix signaling. EMBO J. 2007 Mar 7;26(5):1257-67
• All: 10 reference(s)