Tg(AMELX-LRAP)9Gibs
Transgene Detail

Summary

• Symbol: Tg(AMELX-LRAP)9Gibs
• Name: transgene insertion 9, Carolyn Gibson
• MGI ID: MGI:3716151
• Transgene: Tg(AMELX-LRAP)9Gibs Location: unknown
• Alliance: Tg(AMELX-LRAP)9Gibs page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: 5.5 kb of upstream bovine AMELX gene sequence plus untranslated exon 1 and intron 1 was fused to a bovine LRAP cDNA, and followed by a bovine polyadenylation signal sequence from the bovine AMELX gene. RT-PCR analysis demonstrated that this transgene is expressed in tooth germ by 15 days of fetal age, and western blot analysis demonstrated protein expression in molar tooth germs. Four lines of transgenice mice were produced; in addition to line 9, lines 15, 16 and 22 were made. (J:122916)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:122916 Chen E, et al., The small bovine amelogenin LRAP fails to rescue the amelogenin null phenotype. Calcif Tissue Int. 2003 Nov;73(5):487-95
• All: 3 reference(s)