Tg(AMELX*P70T)2Gibs
Transgene Detail

Summary

• Symbol: Tg(AMELX*P70T)2Gibs
• Name: transgene insertion 2, Carolyn Gibson
• MGI ID: MGI:3716166
• Transgene: Tg(AMELX*P70T)2Gibs Location: unknown
• Alliance: Tg(AMELX*P70T)2Gibs page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(AMELX*P70T)2Gibs expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Amelx (MGI:88005)	Transgene codes for a fusion protein of N-terminal mouse Amelx and C-terminal cow AMELX with a proline to threonine mutation in the cow peptide.
  cattle	AMELX (281620)	Transgene codes for a fusion protein of N-terminal mouse Amelx and C-terminal cow AMELX with a proline to threonine mutation in the cow peptide.

• Mutation details: 5.5 kb of upstream bovine AMELX gene sequence plus untranslated exon 1, intron 1 and part of exon 2 was fused to a partial mouse Amelx cDNA that encodes the M180 isoform from exon 2 to part of exon 5 and a partial bovine AMELX CDNA that encodes the rest of exon 5 and exon 6, and was followed by a bovine polyadenylation signal sequence from the bovine AMELX gene. Site-directed mutagenesis was used to introduce a mutation in exon 6 that results in a proline to threonine substitution at amino acid 70 of the encoded protein. RT-PCR analysis demonstrated that this transgene is expressed in first mandibular molar teeth in 3-5 day old pups. Western blot analysis demonstrated that transgenic protein was expressed in first mandibular molars. Three lines of mice were produced; in addition to line 2, lines 16 and 29 were also created. (J:122914)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:122914 Gibson CW, et al., Transgenic mice that express normal and mutated amelogenins. J Dent Res. 2007 Apr;86(4):331-5
• All: 2 reference(s)