Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Liqun Luo
• MGI ID: MGI:3716464
• Synonyms: Gt(ROSA26)^{ACTB-tdTomato-EGFP}, mT/mG, mTmG, mTomato/mEGFP, R26-mR/mG, R26^TG, R26-X, Rosa26^{flox-mTRed-Stop-flox-mGFP}, ROSA26-mGFP, Rosa26^mTmG, ROSA26-mTmG, Rosa26mTmG^flox, Rosa26R^mTmG, ROSA^mT-mE, Rosa^mTmG, ROSA-mT/mG, ROSA-R/G, TomatoGFP+
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124702
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with a CMV enhancer/chicken beta-actin core promoter (pCA) driving expression of a loxP-flanked, N-terminal membrane-tagged, optimized DsRed fluorescent protein variant (called tandem-dimer-Tomato or tdTomato) sequence followed by a polyadenylation signal. Immediately distal to the second loxP site is an N-terminal membrane-tagged, enhanced green fluorescent protein (EGFP) sequence itself followed by a polyadenylation signal. An frt-flanked neo cassette was also located distal to the expression vector. Red fluorescence is detected in all tissues tested. When mice are bred to Cre expressing mice, the floxed region is excised in Cre-expressing tissue and this allows expression of the EGFP cassette. (J:123053, J:124702)

Disease models

Expression

In Mice Carrying this Mutation:

471 assay results 27 RNA-Seq or microarray experiment(s)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 10 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:124702 Muzumdar MD, et al., A global double-fluorescent Cre reporter mouse. Genesis. 2007 Sep;45(9):593-605
• All: 2135 reference(s)