Afteq2^ISS
QTL Variant Detail

Summary

• QTL variant: Afteq2^ISS
• Name: acute functional tolerance to ethanol QTL 2; ISS
• MGI ID: MGI:3716513
• QTL: Afteq2 Location: unknown Genetic Position: Chr16, cM position of peak correlated region/allele: 14.69 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: ISS

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased functional ethanol tolerance compared to ILS. (J:122921)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:122921

Linkage analysis was performed on 74 LXS recombinant inbred (RI) strains to identify QTLs associated with acute functional tolerance (AFT) to alcohol. The RI strains are derived from ILS (inbred long sleep) and ISS (inbred short sleep) parentals. Animalswere tested between 55 and 95 days of age. An extensive panel of 4,834 polymorphic SNP markers was used for genome scan.

Significant linkage to AFT1 (the difference in blood ethanol concentration after 2 successive regains in balance) mapped to 58 cM onmouse Chromosome 12 near D12Mit8 (LOD=3.54). This locus explains 18% of the variance and is named Afteq1 (acute functional tolerance to ethanol QTL 1). ISS-derived alleles at Afteq1 decreased ethanol tolerance. The QTL interval extends from 84.28 Mb to thetelomere. Slc24a4 at 100.96 Mb is a potential candidate gene for Afteq1. Other potential candidates include Ccnk (52 cM), Yy1 (53 cM), and Slc25a29 (109.27 Mb).

Linkage to AFT2 (the difference in blood ethanol concentration at final regain and initialloss of balance) mapped to 17 cM on mouse Chromosome 16 near D16Mit101 (LOD=2.95) explaining 16% of the phenotypic variance. This locus approached significance and was later detected in multiple loci interactions, so it is designated Afteq2 (acute functionaltolerance to ethanol QTL 2). ILS-derived alleles at Afteq2 increased ethanol tolerance while ISS-derived alleles decreased ethanol tolerance.

Two-way scan analysis detected 6 interacting loci explaining 59% of the phenotypic variance. Afteq1 and Afteq2contribute significantly to this 6-locus model. LS-derived alleles at Afteq1 in conjunction with SS-derived alleles at Afteq2 significantly increased ethanol tolerance (AFT1 values). The two-way scan replicated suggestive loci on chromosomes 1, 11, and 17 from the one-way scan and also dected new suggestive loci on chromosomes 3 and 4.

References

• Original: J:122921 Bennett B, et al., Quantitative trait locus mapping for acute functional tolerance to ethanol in the L x S recombinant inbred panel. Alcohol Clin Exp Res. 2007 Feb;31(2):200-8
• All: 1 reference(s)