Trpm8^tm1Jul
Targeted Allele Detail

Summary

• Symbol: Trpm8^tm1Jul
• Name: transient receptor potential cation channel, subfamily M, member 8; targeted mutation 1, David Julius
• MGI ID: MGI:3716518
• Synonyms: Trpm8^-, trpm8^-(DJ)
• Gene: Trpm8 Location: Chr1:88234457-88318909 bp, + strand Genetic Position: Chr1, 44.57 cM
• Alliance: Trpm8^tm1Jul page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122978
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The self excising ACN cassette was inserted to replace exons 13 and 14. Amino acids 594-661, located in the presumptive cytoplasmic amino-terminal domain, were deleted from the locus, introducing a premature stop codon before and a frame shift after the deleted region. RT-PCR confirmed absence of transcript from mutant trigeminal ganglia. (J:122978)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trpm8 Mutation: 61 strains or lines available

References

• Original: J:122978 Bautista DM, et al., The menthol receptor TRPM8 is the principal detector of environmental cold. Nature. 2007 Jul 12;448(7150):204-8
• All: 38 reference(s)