Trpm8^tm1Apat
Targeted Allele Detail

Summary

• Symbol: Trpm8^tm1Apat
• Name: transient receptor potential cation channel, subfamily M, member 8; targeted mutation 1, Ardem Patapoutian
• MGI ID: MGI:3716558
• Synonyms: Trpm8^-, Trpm8^EGFPf
• Gene: Trpm8 Location: Chr1:88234457-88318909 bp, + strand Genetic Position: Chr1, 44.57 cM
• Alliance: Trpm8^tm1Apat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122963
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Velocigene technology was used to delete sequences encoding amino acid residues 2-29 and insert a farnesylated enhanced GFP gene followed by an SV40polyA tail in frame with the start codon of the endogenous gene. RT-PCR confirmed absence of wild-type transcript in mutants, however, in some samples a truncated splice variant was detected at low levels. (J:122963)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trpm8 Mutation: 61 strains or lines available

References

• Original: J:122963 Dhaka A, et al., TRPM8 is required for cold sensation in mice. Neuron. 2007 May 3;54(3):371-8
• All: 35 reference(s)