Spem1^tm1Wyan
Targeted Allele Detail

Summary

• Symbol: Spem1^tm1Wyan
• Name: spermatid maturation 1; targeted mutation 1, Wei Yan
• MGI ID: MGI:3716924
• Synonyms: Spem1^-
• Gene: Spem1 Location: Chr11:69711697-69712991 bp, - strand Genetic Position: Chr11, 42.89 cM, cytoband B4
• Alliance: Spem1^tm1Wyan page

Morphological and histological analyses of Spem1^tm1Wyan/Spem1^tm1Wyan mouse testes and sperm

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:120937
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace the 250 bp long 5' UTR and exons 1-3, except for the last 88 bp of exon 3, with a pgk-hprt cassette. Absence of transcript and protein was confirmed in mutant testis by Northern blot and Western blot analysis, respectively. (J:120937)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spem1 Mutation: 17 strains or lines available

References

• Original: J:120937 Zheng H, et al., Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation, and male infertility. Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6852-7
• All: 3 reference(s)