St14^{Gt(RST485)Byg}
Gene trapped Allele Detail

Summary

• Symbol: St14^{Gt(RST485)Byg}
• Name: suppression of tumorigenicity 14 (colon carcinoma); gene trap RST485, BayGenomics
• MGI ID: MGI:3717153
• Synonyms: St14^e16beta-gal
• Gene: St14 Location: Chr9:31000698-31043149 bp, - strand Genetic Position: Chr9, 16.61 cM
• Alliance: St14^{Gt(RST485)Byg} page

Mutation origin

• Mutant Cell Line: RST485
• Germline Transmission: Earliest citation of germline transmission: J:108567
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2TMpfs Vector Type: secretory trap
• Mutation details: Insertion of a genetrap vector placed into intron 16 a pGT1TM construct containing the intron from the engrailed 2 gene upstream of the gene encoding beta-galactosidase/neomycin-phosphotransferase fusion protein. (J:108567)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RST485	CC199786 ; (MGI Seq Detail)	5' RACE	Chr9:31006830-31007002 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  8 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any St14 Mutation: 45 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:108567 List K, et al., Delineation of matriptase protein expression by enzymatic gene trapping suggests diverging roles in barrier function, hair formation, and squamous cell carcinogenesis. Am J Pathol. 2006 May;168(5):1513-25
• All: 4 reference(s)