Tg(Thy1-PSEN1*A246E)2Vln
Transgene Detail

Summary

• Symbol: Tg(Thy1-PSEN1*A246E)2Vln
• Name: transgene insertion 2, Fred Van Leuven
• MGI ID: MGI:3717624
• Synonyms: Ps1 A246E, Ps1/A246E, PS1[A246E], PS1mut
• Transgene: Tg(Thy1-PSEN1*A246E)2Vln Location: unknown
• Alliance: Tg(Thy1-PSEN1*A246E)2Vln page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-PSEN1*A246E)2Vln expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PSEN1 (5663)

  Psen1

  (MGI:1202717)

  Transgene codes for a protein with a alanine to glutamic acid mutation.

• Mutation details: cDNA encoding the human PSEN1 sequence containing the A246E mutation was ligated into the mouse Thy1 gene. In the final construct, coding sequences and introns 1 and 2 of the Thy1 gene are replaced by the mutant PSEN1 sequence, with the 5' neuron-specific control sequences of Thy1 intact. Linearized minigenes are microinjected into prenuclear FVB/N embryos. Mutant PSEN1 mRNA and protein are detected in total brain extracts by Northern and Western blotting, respectively. (J:64209, J:100968)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

J:100968 discusses generation of trangenic PSEN1[A246E] mice, referencing J:64209 as one paper on the method. Since the transgenic mice are apparently identical, it was assumed that new transgenic mice were not created, but mice are actually the ones referenced in J:64209. -rbabiuk 8/9/2007

References

• Original: J:64209 Dewachter I, et al., Aging increased amyloid peptide and caused amyloid plaques in brain of old APP/V717I transgenic mice by a different mechanism than mutant presenilin1. J Neurosci. 2000 Sep 1;20(17):6452-8
• All: 12 reference(s)