Tg(Gfap-TGFB1)64Lms
Transgene Detail

Summary

• Symbol: Tg(Gfap-TGFB1)64Lms
• Name: transgene insertion 64, Lennart Mucke
• MGI ID: MGI:3717676
• Synonyms: GFAP/TGF-B1, pTGF-B1
• Transgene: Tg(Gfap-TGFB1)64Lms Location: unknown
• Alliance: Tg(Gfap-TGFB1)64Lms page

Transgene origin

• Strain of Origin: (BALB/c x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gfap-TGFB1)64Lms expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  pig, domestic	TGFB1 (397078)

• Mutation details: The construct contains a 1.35 kb porcine transforming growth factor beta 1 (TGFB1) cDNA inserted into the first exon of the mouse glial fibrillary acidic protein (Gfap) gene, making a fusion gene The TGFB1 cDNA has been mutated to encode serines at positions 223 and 225, instead of cysteines, to ensure secretion of a bioactive peptide. The linear construct was microinjected into (BALB/c x SJL)F2 one-cell stage embryos. Line 64 founder mice expressed low levels of TGFB1 mRNA in the brain. Line 115 was also generated and shows very similar properties. (J:27781)

Disease models

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Homozygous trangenic mice develop severe hydrocephalus within 7-20 days after birth and exhibit dwarfism relative to non-transgenic littermates. Mice develop seizures, tremors, incoordination, and hunched postures. Only 1% of heterozygotes developed hydrocephalus.

References

• Original: J:27781 Wyss-Coray T, et al., Increased central nervous system production of extracellular matrix components and development of hydrocephalus in transgenic mice overexpressing transforming growth factor-beta 1. Am J Pathol. 1995 Jul;147(1):53-67
• All: 20 reference(s)