Bsx^tm2Aoh
Targeted Allele Detail

Summary

• Symbol: Bsx^tm2Aoh
• Name: brain specific homeobox; targeted mutation 2, Akihira Ohtoshi
• MGI ID: MGI:3719245
• Synonyms: Bsx^lacZ
• Gene: Bsx Location: Chr9:40785423-40791353 bp, + strand Genetic Position: Chr9, 21.65 cM
• Alliance: Bsx^tm2Aoh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:123638
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Targeting took place in PC3 ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. A targeting vector containing lacZ sequence and a floxed neo cassette was inserted by homologous recombination in ES cells. The neo cassette was subsequently removed upon transmission from male chimeric mice, resulting in the deletion of the homeodomain and expression of the lacZ reporter. Absence of endogenous transcript was confirmed by RT-PCR. (J:123638)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bsx Mutation: 19 strains or lines available

References

• Original: J:123638 McArthur T, et al., A brain-specific homeobox gene, Bsx, is essential for proper postnatal growth and nursing. Mol Cell Biol. 2007 Jul;27(14):5120-7
• All: 1 reference(s)