Pum2^Gt(XE772)Byg
Gene trapped Allele Detail

Summary

• Symbol: Pum2^Gt(XE772)Byg
• Name: pumilio RNA-binding family member 2; gene trap XE772, BayGenomics
• MGI ID: MGI:3719788
• Synonyms: Pum2^XE772
• Gene: Pum2 Location: Chr12:8724134-8802581 bp, + strand Genetic Position: Chr12, 3.92 cM, cytoband A1.3
• Alliance: Pum2^Gt(XE772)Byg page

Mutation origin

• Mutant Cell Line: XE772
• Germline Transmission: Earliest citation of germline transmission: J:123531
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The pGT1Lxf gene trap vector containing a beta-geo inserted into intron 10. A 5.1 kb transcript was detected with the expected size of the fusion between the truncated locus (exons 1-10) and the beta-geo. Because the transcript was disrupted before the highly conserved PUMILIO homology domain, a functional protein is unlikely. (J:123531)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE772	CC183330 ; (MGI Seq Detail)	5' RACE	Chr12:8771723-8774174 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  97 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pum2 Mutation: 104 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:123531 Xu EY, et al., A gene trap mutation of a murine homolog of the Drosophila stem cell factor Pumilio results in smaller testes but does not affect litter size or fertility. Mol Reprod Dev. 2007 Jul;74(7):912-21
• All: 10 reference(s)