Tg(Thy1-App*R609D*K612E)4Vln
Transgene Detail

Summary

• Symbol: Tg(Thy1-App*R609D*K612E)4Vln
• Name: transgene insertion 4, Fred Van Leuven
• MGI ID: MGI:3720070
• Synonyms: t/RK/F/4
• Transgene: Tg(Thy1-App*R609D*K612E)4Vln Location: unknown
• Alliance: Tg(Thy1-App*R609D*K612E)4Vln page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-App*R609D*K612E)4Vln expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	App (MGI:88059)	Transgene codes for a protein with arginine to aspartic acid and lysine to glutamic acid mutations.

• Mutation details: Murine APP cDNA modified to have 2 mutations, Arg609 to Asp and Lys612 to Glu, was inserted into the mouse Thy1 gene, replacing a 1.5 kb fragment containing part of the sequence spanning from within exon 2 into exon 4. The thymus-specific elements in intron 3 were deleted in the construct. The construct was microinjected into fertilized 1.5-day-old pre-nuclear embryos from FVB females. RT-PCR verified expression of the transgene in the brain of heterozygotes at ~50% of endogenous App message. Line 4 was established. (J:32213)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:32213 Moechars D, et al., Expression in brain of amyloid precursor protein mutated in the alpha-secretase site causes disturbed behavior, neuronal degeneration and premature death in transgenic mice. EMBO J. 1996 Mar 15;15(6):1265-74
• All: 1 reference(s)