Tg(Thy1-App*R609D*K612E)6Vln
Transgene Detail
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Symbol: |
Tg(Thy1-App*R609D*K612E)6Vln |
Name: |
transgene insertion 6, Fred Van Leuven |
MGI ID: |
MGI:3720071 |
Synonyms: |
t/RK/F/6 |
Transgene: |
Tg(Thy1-App*R609D*K612E)6Vln Location: unknown
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Alliance: |
Tg(Thy1-App*R609D*K612E)6Vln page
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Transgene Type: |
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Transgenic (Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(Thy1-App*R609D*K612E)6Vln expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Note |
mouse |
App (MGI:88059) |
Transgene codes for a protein with arginine to aspartic acid and lysine to glutamic acid mutations. |
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Mutation details: Murine APP cDNA modified to have 2 mutations, Arg609 to Asp and Lys612 to Glu, was inserted into the mouse Thy1 gene, replacing a 1.5 kb fragment containing part of the sequence spanning from within exon 2 into exon 4. The thymus-specific elements in intron 3 were deleted in the construct. The construct was microinjected into fertilized 1.5-day-old pre-nuclear embryos from FVB females. RT-PCR verified expression of the transgene in the brain of heterozygotes at ~50% of endogenous App message. Line 6 was established.
(J:32213)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Original: |
J:32213 Moechars D, et al., Expression in brain of amyloid precursor protein mutated in the alpha-secretase site causes disturbed behavior, neuronal degeneration and premature death in transgenic mice. EMBO J. 1996 Mar 15;15(6):1265-74 |
All: |
1 reference(s) |
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