Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc
Transgene Detail

Summary

• Symbol: Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc
• Name: transgene insertion 837, Oksana Lockridge
• MGI ID: MGI:3720257
• Synonyms: G117H BChE, M844T
• Transgene: Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc Location: unknown
• Alliance: Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc page

Transgene origin

• Strain of Origin: C57BL/6 x (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BCHE (590)

  Bche	(MGI:894278)

• Mutation details: The human G117H BChE sequence (encoding the 28 amino acid signal peptide and 574 amino acid full-length protein) was modified with a single His for Gly amino acid mutation at codon 117 (G117H). To enhance expression, a chimeric intron (consisting of a human beta-globin gene and the branch and 3' acceptor site from the intron of an immunoglobulin gene heavy chain variable region) was placed between the promoter and human G117H BChE sequence. A FLAG epitope tag (encoding amino acids DYKDDDDK) was placed at the carboxy-terminus of the human G117H BChE. The transgene also contained the human elongation factor 1-alpha poly(A) sequence at the 3' end. This transgene was microinjected into fertilized [C57BL/6 x (C57BL/6xSJL)F1] eggs. Founder mice (line M837T) were bred to B6SJL/F1Tac mice. All tested tissues express G117H BChE enzyme activity. In addition, 2 other stable lines M844T and M853T were generated, with detailed analysis of M844T described. (J:89332)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:89332 Wang Y, et al., Resistance to organophosphorus agent toxicity in transgenic mice expressing the G117H mutant of human butyrylcholinesterase. Toxicol Appl Pharmacol. 2004 May 1;196(3):356-66
• All: 1 reference(s)